Hydrogel locomotion regulated by light and electric fields

by Thamarasee Jeewandara , Phys.org

Hydrogel locomotion regulated by light and electric fields
Charge reversal of the spiropyran-PNIPAM polymers. (A) Schematic of charge reversal of the SP/SP(SO3) spiropyran-PNIPAM hydrogel. The net charge of spiropyran and sulfonated spiropyran molecules changes from +1 to 0 and from 0 to −1, respectively. (B)Potential values of the SP/SP(SO3) spiropyran-PNIPAM solutions with different SP/SP(SO3) molar ratios in acidic MeOH/water (4:1, v/v) (pH 2.32) after overnight incubation in the dark and exposure to 450 nm light (0.95 mW/cm2) for 25 min. The changes in ζ-potential Δζ are obtained from the original potential distribution curves. (C) Repeated measurements of ζ over five light-dark cycles for the spiropyran-PNIPAM polymers containing SP, SP(SO3), and SP/SP(SO3) with the molar ratio of 1:1. The light and dark periods take 25 min and two hours, respectively. (D and E) Normalized ζ-potential (ζ/Δζ) of the PNIPAM polymers with different SP/SP(SO3) molar ratios (1:0, 2:1, 1:1, 1:2, and 0:1) versus time of light irradiation (D) and subsequent recovery in dark (E) (the reference curves are fitted with an exponential function; original ζ-potential curves are shown). (F) Normalized ultraviolet (UV)–visible absorbance intensity of SP and SP(SO3) molecules (0.1 mM) at 432 nm (protonated merocyanine form) as a function of solution pH with sigmoidal fitting curves indicating pKa values . a.u., arbitrary units. Credit: Science Advances, doi: 10.1126/sciadv.adi4566

Materials scientists aim to develop autonomous materials that function beyond stimulus responsive actuation. In a new report in Science Advances, Yang Yang and a research team in the Center for Bioinspired Energy Science at the Northwestern University, U.S., developed photo- and electro-activated hydrogels to capture and deliver cargo and avoid obstacles on return.

To accomplish this, they used two spiropyran monomers (photoswitchable materials) in the hydrogel for photoregulated charge reversal and autonomous behaviors under a constant electric field. The photo/electro-active materials could autonomously perform tasks based on constant external stimuli to develop intelligent materials at the molecular scale.

Bioengineering a charged hydrogel

Soft materials with life-like functionality have promising applications as intelligent, robotic materials in complex dynamic environments with significance in human-machine interfaces and biomedical devices. Yang and colleagues designed a photo- and electro-activated hydrogel to capture and deliver cargo, avoid obstacles, and return to its point of departure, based on constant stimuli of visible light and applied electricity. These constant conditions provided energy to guide the hydrogel.

The research team covalently integrated spiropyran moieties with varying substituents into the constructs to regulate the net charge of the soft materials. They used finite element simulations to guide the design and movement of the charged hydrogels and engineer 3D surface profiles to maximize the dielectrophoretic effect. Yang and the team further studied the scope of electroactive locomotion and photoactuation in the spiropyran hydrogels.

Geometric stability of the spiropyran hydrogel discs with different diameters under light irradiation. Credit: Science Advances, doi: 10.1126/sciadv.adi4566

Charge reversal of spiropyran-functionalized polymers

Yang and colleagues used two different spiropyran molecules with different net charges. They synthesized each of the molecules with a polymerizable methacrylate group based on existing reports.

They incorporated different ratios of the spiropyran molecules into N-isopropylacrylamide polymer chains (PNIPAM) to form hydrogels. In this instance, they tuned the charge reversal functionalities using copolymers of the spiropyran structural units to show photoswitchable potential and charge reversible behaviors with tunable charge. The scientists tuned the charge reversal time by changing the ratio of the two spiropyran moieties, without changing the switching and recovery rates.

Photo-activated electroactive motion of the spiropyran-PNIPAM hydrogels

Based on charge reversal behavior of the polymers, Yang’s team photoregulated the electroactive hydrogels by using a crosslinker to prepare them.

At first, the team could positively charge the hydrogel to move towards the cathode under a direct current electric field, where the positive charge transferred from the spiropyran moieties into the hydrogel network. Thereafter, the permanently bound sulfonate groups on the polymer chain made the net charge of the construct negative, allowing the negatively charged hydrogel to navigate back to the anode.

Autonomous obstacle avoidance of the low-k barriers and return function by the three-arm hydrogel object under constant electric field and light irradiation. Credit: Science Advances, doi: 10.1126/sciadv.adi4566

The team studied the photoregulated electroactive locomotion speeds of the hydrogel disks across multiple light-dark cycles to examine their locomotion speed, and determined the relationship between the charge and speed of the hydrogel disks. They based this on the balance between the electrostatic force and hydrodynamic drag force, where the higher applied voltage and larger diameter of the hydrogel disks delivered higher locomotion speed. Such polymeric devices are well-suited to capture and deliver cargo through autonomous hunting.

Capturing and delivering cargo

Yang and colleagues explored the cargo delivery potential of the constructs by engineering simple disk-shaped spiropyran-PNIPAM hydrogels and sphere-shaped constructs embedded with nanoparticles as cargos. The strong dielectrophoretic force allowed the materials to undergo autonomous hunting and picking up functions.

Based on simulations, Yang and colleagues formed a 3-arm spiropyran PNIPAM hydrogel object using photoinitiated free radical polymerization with superior capture capability of the cantilever arms. When uncharged, the electric field gradient around the hydrogel vanished, enabling autonomous cargo release during charge reversal. The cargo release also occurred by turning off the electric field

Automatically avoiding obstacles

The research team showed how materials with a high dielectric constant induced an attractive electrophoretic force, and materials with a lower dielectric constant exerted a repulsive electrophoretic force on the adjacent charged hydrogel object.

Using finite element calculations, they showed the possibility of low dielectric constants to guide the charged hydrogel through obstacles. Under constant stimuli of the electric field and light irradiation, the hydrogel automatically bypassed barriers and traveled back after charge reversal, without human intervention.

Outlook

In this way, Yang and colleagues designed a photo- and electroactive hydrogel that can cargo capture and deliver, as well as avoid obstacles under constant external stimuli. The scientists used two different ratios of spiropyran moieties in the hydrogel and facilitated the net charge in the chemically random network to be tunable under irradiation with blue light. This enabled photoregulated, electroactive motion with autonomous behavior under the direction of light and electricity.

The autonomous soft matter products elegantly captured and delivered cargo while avoiding obstacles with applications suited for scenarios to ensure the safety of monitoring a situation from afar—for instance, where human intervention is impractical. These new biomaterials with autonomous functionality can be resourcefully engineered using environmentally sensitive electrostatic interactions and photoactuation in soft materials.

More information: Yang Yang et al, Autonomous hydrogel locomotion regulated by light and electric fields, Science Advances (2023). DOI: 10.1126/sciadv.adi4566

Anne Helene Gelebart et al, Making waves in a photoactive polymer film, Nature (2017). DOI: 10.1038/nature22987

Journal information: Science Advances  Nature 

© 2023 Science X Network